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Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282


ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

Articoli con TAG: componente monoclonale

Studio sulla presenza di componenti monoclonali in pazienti con infezione da SARS-CoV-2: dati preliminari
Presence of monoclonal components in SARS-CoV-2 patients: preliminary data
<p>Introduction: electrophoresis of serum proteins (EF) is indicated for the identification and monitoring of monoclonal components (CM). It has been shown that interleukins play a role in the differentiation of B cells in plasma cells producing immunoglobulins; it has been also demonstrated that COVID-19 patients show a higher prevalence of CM in comparison to the general population. The aim of this work is to retrospectively evaluate the presence of CM in patients hospitalized with COVID-19, compared to a population of patients admitted in non-COVID-19 wards.<br />Methods: EF was performed in the two groups of patients (COVID positive and negative) using capillary electrophoresis. Patients with previous plasma cell dyscrasias have been excluded.<br />Results: the results show that in the COVID positive group, the incidence of CM is statistically higher compared to the COVID negative group (39.7% versus 13.3%). In one patient, the CM was no longer detectable when the swab became negative.<br />Conclusions: the study confirmed that the viral infection produces detectable CM, probably transitory as shown by a case index. The pathogenesis of the phenomenon could be explained by the cytokine stimulus on B cells and by the interaction of the virus with the lymphocyte ACE 2 receptor. Larger studies are needed to confirm the presented data.</p>
Biochimica Clinica ; 17(1)
Contributi Scientifici - Scientific Papers
Misura e identificazione delle componenti monoclonali plasmatiche: risultati di uno studio multicentrico internazionale
The measurement and the identification of plasma monoclonal proteins: results from an international multicentre study.
<p>The measurement of monoclonal proteins (MP) is basic for diagnosis, risk stratification, and evaluation of the response to the therapeutic treatment of plasma cells dyscrasias and lymphoproliferative disorders. Quality specifications of the MP measurement have been evaluated in a multicentre study involving 14 clinical laboratories and 2 In vitro Diagnostics Companies across three continents. Aliquots of human serum pools with normal, hypo- and hyper &gamma;-globulinemia spiked with different amounts of therapeutic monoclonal drugs migrating in the &gamma;-globulin zone, mimicking a MP and serum aliquots with a MP migrating in &beta;-globulin zone were distributed to participants. Two articles, published in Clinical Chemistry and Laboratory Medicine, reported detailed results emerging from the study; the most significant have been condensed in the present paper. The MP concentration, the amount of the co-migrating proteins, especially the polyclonal immunoglobulins background, and the MP migration pattern significantly affect accuracy and precision of the MP quantification. When MP is &lt;1 g/L, the unacceptable imprecision and the loss of accuracy, strongly discourage to report any numeric data, even though the presence of MP in the serum protein electrophoresis (SPE) must be reported qualitatively. The two gating techniques, namely perpendicular drop (DP) and tangent skimming (TS), lead to overestimation and underestimation of small MP, respectively. All the tested SPE methods, two agarose gel- and one capillary zone electrophoresis, detected 100% of MP &ge;1 g/L; as expected, the limit of detection of immunofixation and immunosubtraction were moderately more sensitive. The overall mean intra-laboratory coefficient of variation (CV) for MP ranging 1-10 g/L was found around 5.0%; thus, the monitoring of changes in MP level over time within the same laboratory is considered reliable provided that the intensity of the polyclonal background remains stable.</p>
Biochimica Clinica ; 44(3) 270-278
Opinioni - Opinions
Interferenze da farmaci biologici: un caso di accumulo di Bevacizumab
Interference by biological anti-cancer drugs: the case of Bevacizumab
<p>We report a case of interference with electrophoretic (CZE) and immunofixation (IFE) techniques after repeated cycles of a biological anti-cancer drug. A man, with colon neoplasia, underwent a prolonged therapy with Bevacizumab and other chemoterapeutic agents from September 2013 until May 2015. Before therapy, the electrophoretic pattern was normal while, during treatment, it showed a modification suggestive of the presence of a monoclonal component (MC) that was possible to type (IgG <span style="font-family:symbol; font-size:11.0pt">k</span>) and to quantify (8 g/L) after 22 cycles of therapy. The cooperation with clinicians and our study about biological drugs, allowed us to recognize this MC as an interference due to the accumulation of Bevacizumab in the serum of the patient. The laboratory report of a MC would have involved the patient in further procedures that are invasive, cost and time expensive. This case emphasizes the importance of a strict collaboration between physicians and the clinical laboratory in the management of patients treated with biological drugs.</p>
Biochimica Clinica ; 42(1) e05-e07
Casi clinici - Case report