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Editor-in-chief
Maria Stella Graziani
Deputy Director
Martina Zaninotto
Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali
EIC Assistant
Francesco Busardò
International Advisory Board
Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada
Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano
Responsible Editor
Giuseppe Agosta
Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it
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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091
BC: Articoli scritti da A. Tinello
Comparison of Sysmex UF-100 and UF-1000i with urine culture for the diagnosis of urinary tract infections
<p>Urinary tract infections (UTI) are a common clinical condition. The gold standard for diagnosis is still the bacterial culture, even though a large proportion of evaluated samples are negative. Unnecessary cultures can be reduced by an effective screening test. The aim of this study was to compare two cytometers for rapid diagnosis of UTI. Using 209 urine samples submitted to our laboratory for microbiological examination, we evaluated the analytical performance of the new urine cytometer UF-1000i in comparison with the previous generation analyzer UF-100 (both from Sysmex). We compared bacteria (BACT) and leukocyte (WBC) counts performed by UF-1000i and UF-100 with colony-forming units (CFU) quantification on citrate lactose electrolytes deficient (CLED) agar to assess the best cutoff values. Moreover, a correlation between BACT and WBC quantification performed by the two instruments was carried out. In comparison with 1x108 CFU/L, cut-off values of 1.25x108 BACT/L and 4x107 WBC/L by using UF-1000i, and 3x109 BACT/L and 3.5x107 WBC/L by using UF-100 were obtained, respectively. While WBC quantification by UF-1000i and UF-100 showed a strong correlation (r=0.98), BACT quantification displayed a poor correlation (r=0.59).</p>
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