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Giuseppe Agosta

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da T. Rubeca

Valutazioni preliminari per la proposta di un unico dispositivo di campionamento per la ricerca dell’emoglobina su materiale fecale
Preliminary assessments for a standard sampling device for fecal hemoglobin detection
S. Rapi  |  C.G. Fraser  |  F. Cellai  |  M. Berardi  |  T. Rubeca  | 
<p>Sampling of feces is&nbsp;strongly affected by the lack of harmonisation, with differences up to 20 times in the mass collected for immunological&nbsp;tests for fecal hemoglobin (Hb) used in colorectal cancer screening. Aim of this study was to acquire information on&nbsp;fecal sampling and on the interaction between feces and analytical methods to obtain a reference design for a&nbsp;sampling dipstick. Bias and imprecision of sample collection dipsticks were estimated using gravimetry. Dissolution&nbsp;times of feces were monitored throughout the study. The effect of increasing amount of feces on Hb concentrations&nbsp;was investigated in saline and buffers of different manufacturers using a single analytical method (OC-Sensor, Eiken&nbsp;Chemical Co.). Fecal mass recovered with different devices ranged from 56 to 121% of declared amount (CV range:&nbsp;8.6&divide;31.1%). Time of dissolution up to 2 h was observed when lumps of materials were collected. In saline a rapid&nbsp;decrease of Hb values was observed, which was related to the overall amount of feces. Increased Hb values were<br />observed by adding feces to manufacturers&rsquo; buffers. Solubilisation time, bias and imprecision of sampling of feces&nbsp;were related to device design. Analytical methods are designed to use specific ratios between feces and buffers. The&nbsp;introduction of a standard dipstick design to reduce preanalytical variability may represent a crucial step for fecal test&nbsp;harmonization.</p>
Biochimica Clinica ; 39(6) 563-567
Contributi scientifici - Scientific Papers