Member area login
You don't have or don't remember the password!
Click Here
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282


ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da G. Righetti

Valutazione delle caratteristiche analitiche di un nuovo strumento per l’elettroforesi capillare delle proteine sieriche
Evaluation of the analytical performance of a new capillary electrophoresis system for serum protein analysis
<p>Electrophoresis techniques are used in clinical laboratories to detect, type and quantify monoclonal&nbsp;components (MC), and are therefore of pivotal importance in the management of patients with monoclonal&nbsp;gammopathies. Capillary electrophoresis (CE) is an attractive alternative to agarose gel electrophoresis (AGE)&nbsp;because it offers a higher degree of automation without requiring the use of toxic reagents. Aim of this study was to&nbsp;evaluate analytical characteristics of a recently marketed instrument for CE, comparing it with both a well established&nbsp;CE and with AGE. The new system (V8, Helena Biosciences) was compared for MC detection with Capillarys (Sebia)&nbsp;and for typing with both the AGE Hydrasys system (Sebia) and the immunosubtraction technique on Capillarys. All&nbsp;the evaluated analytical characteristics were related to MC investigations, i.e. detection, typing and quantification. The&nbsp;interference effect of some substances and the carryover were also investigated. For MC detection, an agreement of&nbsp;98.5% has been demonstrated between the two CE systems; the discordant samples show a MC &lt;1 g/L. The limit of&nbsp;detection for a MC was 0.5 g/L. A total agreement in the typing of MC &gt;5 g/L between the two CE systems was&nbsp;observed. V8 was able to correctly characterize 18 MC with concentration &lt;5 g/L out of 20 samples typed by AGE&nbsp;immunofixation. V8 can quantify MC with elevated precision (CV 3,5% and 1,2% for <span style="font-family:symbol">b</span>- and <span style="font-family:symbol">g</span>-zone MC, respectively)&nbsp;and the measurement is linear till &lt;1 g/L. Fibrinogen and contrast media appear in the CE patterns as homogeneous&nbsp;bands MC like. From these results, we conclude that the V8 analytical characteristics are fully adequate for the&nbsp;system use in clinical laboratories for managing patients with monoclonal gammopathies.</p>
Biochimica Clinica ; 38(4) 295-301
Contributi scientifici - Scientific Papers
Sei casi di gammopatia monoclonale IgD
Six cases of IgD monoclonal components.
Biochimica Clinica ; 35(5) 417
E' possibile utilizzare la determinazione della lipocalina associata alla gelatinasi dei neutrofili (NGAL) nella valutazione della nefrotossicità da bisfosfonati?
Biochimica Clinica ; 35(4) 331