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Maria Stella Graziani

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Martina Zaninotto

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Ferruccio Ceriotti
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Matteo Vidali

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Francesco Busardò

International Advisory Board Khosrow Adeli Canada
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Giuseppe Agosta

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da S. Rapi

Caratterizzazione dei calcoli urinari mediante spettroscopia Raman e spettroscopia infrarossa in trasformata di Fourier a confronto col metodo chimico-colorimetrico
Characterization of urinary stones using Raman and Fourier transform infrared spectroscopy compared to a chemical colorimetric method
<p>Introduction: Chemical-colorimetric methods for kidney stones identification are widely adopted in most routine laboratories due to low cost with no need of instrumental analysis. Actually, kidney stones are well characterized with the Fourier transform infrared spectroscopy combined with Attenuated Total Reflection (ATR/FT-IR) that allows quick identification of chemical compounds into solid samples. Furthermore, Raman spectroscopy is under investigation as a complementary technique to ATR/FT-IR.<br />Methods: the comparison between chemical-colorimetric method and ATR/FT-IR and Raman spectroscopy has been performed evaluating standards (n=60) and urinary stones (n=89), after morphological and chemical characterization by Raman spectroscopy.<br />Results: identification rate of low quantities in standards (5% and 10% respectively) for the three methods were as follow: chemical-colorimetric method, 20% and 40%; Raman spettroscopy, 87% and 90%; ATR/FT-IR, 53% and 63%. The comparison of the 89 urinary stones revealed that chemical-colorimetric method results differ from spectroscopic technique identifications particularly for oxalate ions, phosphate and uric acid (15 samples out of 89). The agreement between the investigated methods is more than 80% in kidney stones with one prevalent compound (56 samples). In the other samples characterized by two or more compounds (33 samples), discordant identifications between methods were evaluated and discussed.<br />Discussion: ATR/FT-IR and Raman spectroscopy present many advantages in terms of identification of the most representative compounds of kidney stones; therefore, they represent a valid solution for a second level laboratory that receives a large number of samples and should examine them with the most adequate technological support.</p>
Biochimica Clinica ; 45(4) 365-374
Contributi Scientifici - Scientific Papers
Interferenza da anticorpi eterofili e anti fosfatasi alcalina in un paziente sottoposto ad intervento cardiochirurgico di David
Etherophilic and anti-alkaline phosphatase antibodies interference in a patient undergoing David procedure
F. Balboni  |  M. Biagioli  |  V. Comito  |  S. Rapi  |  M. Vannucci  | 
<p>We describe the case of a 47 years old male patient who underwent cardiac surgery for valve sparing root replacement (known as David procedure), in September 2018. In the post-operative period and during the rehabilitation stage, the patient was constantly monitored with the measurement of cardiac troponin I (cTnI), performed in different hospital facilities using different cTnI analytical methods. In the rehabilitation clinic, cTnI [measured with Access AccuTnI+3 method (Beckman Coulter)] was found steadily raising, so the patient was readmitted to the hospital to undergo a coronary angiography. The coronary arteries were found free from critical lesions and the cTnI [measured with Advia Centaur cTnI Ultra method, (Siemens Healthcare)] was repeatedly negative. This finding rose the suspect of an analytical interference in the Access AccuTnI+3 method. This was actually the case: heterophile and anti-alkaline phosphatase antibodies were demonstrated in the patient sample; they both were responsible for the elevated values of cTnI.</p>
Biochimica Clinica ; 44(2) S097-S100
Casi Clinici - Case Report
Valutazioni preliminari per la proposta di un unico dispositivo di campionamento per la ricerca dell’emoglobina su materiale fecale
Preliminary assessments for a standard sampling device for fecal hemoglobin detection
S. Rapi  |  C.G. Fraser  |  F. Cellai  |  M. Berardi  |  T. Rubeca  | 
<p>Sampling of feces is&nbsp;strongly affected by the lack of harmonisation, with differences up to 20 times in the mass collected for immunological&nbsp;tests for fecal hemoglobin (Hb) used in colorectal cancer screening. Aim of this study was to acquire information on&nbsp;fecal sampling and on the interaction between feces and analytical methods to obtain a reference design for a&nbsp;sampling dipstick. Bias and imprecision of sample collection dipsticks were estimated using gravimetry. Dissolution&nbsp;times of feces were monitored throughout the study. The effect of increasing amount of feces on Hb concentrations&nbsp;was investigated in saline and buffers of different manufacturers using a single analytical method (OC-Sensor, Eiken&nbsp;Chemical Co.). Fecal mass recovered with different devices ranged from 56 to 121% of declared amount (CV range:&nbsp;8.6&divide;31.1%). Time of dissolution up to 2 h was observed when lumps of materials were collected. In saline a rapid&nbsp;decrease of Hb values was observed, which was related to the overall amount of feces. Increased Hb values were<br />observed by adding feces to manufacturers&rsquo; buffers. Solubilisation time, bias and imprecision of sampling of feces&nbsp;were related to device design. Analytical methods are designed to use specific ratios between feces and buffers. The&nbsp;introduction of a standard dipstick design to reduce preanalytical variability may represent a crucial step for fecal test&nbsp;harmonization.</p>
Biochimica Clinica ; 39(6) 563-567
Contributi scientifici - Scientific Papers