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Editor-in-chief
Maria Stella Graziani
Deputy Director
Martina Zaninotto
Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali
EIC Assistant
Francesco Busardò
International Advisory Board
Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada
Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano
Responsible Editor
Giuseppe Agosta
Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it
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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091
BC: Articoli scritti da A. Raglio
Automated screening of bacterial meningitis by cytofluorimetric analysis of cerebrospinal fluid: preliminary results
<p>This study was planned to assess the diagnostic performance of the automated urine particle analyzer Sysmex UF-1000i for the rapid screening of cerebrospinal fluid (CSF) in patients with suspected meningitis. Cytometric analyses with either optical microscopy (OM) or UF-1000i, along with assessment of glucose and protein in CSF, were performed on 101 consecutive CSF of patients with suspected meningitis. In 50 out of 101 samples, cultural analysis was also performed with different culture media. Four different diagnostic combination were developed, with different mix of the tested parameters. A high correlation was found between OM and UF-1000i (r=0.99; mean bias, -4.9/μL). The diagnostic agreement was 0.90 in adults and 0.97 in children. The diagnostic agreement between CSF culture and bacterial count by UF-1000i was 0.98, with 1.00 sensitivity and 0.98 specificity. Results showed that the diagnostic combination based on CSF glucose and total proteins, cytometric analysis (leukocyte count ± neutrophilia) and bacterial count on UF1000i exhibited the best performance when compared with microbiological examination (area under ROC curve, 1.00). In conclusion, the results of this study show that the combination of two rapid clinical chemistry tests such as glucose and total proteins with UF-1000i analysis could represent a valid approach for supporting more complex analyses or even for replacing OM and CSF culture during stat examination and to achieve a quick detection of central nervous system infections.</p>
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