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Editor-in-chief
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da L. Politi

Armonizzazione in Medicina di Laboratorio
Harmonization in Laboratory Medicine
F. Ceriotti \| M. Panteghini \| A. Tosetto \| V. Valentini \| L. Politi \| R. Rolla \| T. Guastafierro \| T. Köken \| E. Capoluongo \| C. Mazzaccara \| V. D'Argenio \| V. D'Argenio \| G. Lippi \| M. Plebani \| D. Giavarina \| M. Berardi \| A survey on sample matrix and preanalytical management in clinical laboratories \| D. Bozzato \| G. Messeri \| M. Zaninotto \| M. Vidali \| A. Padoan \| G. Parigi \| A. Clerico \| L. Sciacovelli \| M. Ciaccio \| G.L. Salvagno \| G. Barberio \| G. Barberio \| G.L. Salvagno \| M. Pepe \| M. Panteghini \| F. Braga \| G. Gessoni \| M. Montagnana \| N. Doğan \| M. Barberis \| M. Barberis \| A. Marchetti \| F. Borrillo \| L. Bonfanti \| P.M. Ness \| G. Messeri \| S. Nannini \| J. Queraltò \| M. Zaninotto \| A. Mosca \| BM. Henry \| G. Santini \| A. Coglianese \| V. D'Argenio \| E. Fiorio \| L. Crinò \| M. A. V. Willrich \| A. Modenese \| M. Berardi \| G. Nordera \| M. Girelli \| R. Tomaiuolo \| D. Giavarina \| R. Dittadi \| L. Pighi \| V. Guaraldo \| G. Bambagiotti \| E. Franceschini \| R. Danesi \| M. Locatelli \| F. Balboni \| D. Cosseddu \| M. Savoia \| S. Bernardini \| C. Domenichini \| M. Lamonaca \| M. Perrone \| M. Perrone \| per il Gruppo di Studio Intersocietario SIBioC-SIPMeL Diabete Mellito \| P. Pradella \| A. Padoan \| M.T. Sandri \| L. Belloni \| A. D'Avolio \| T. Trenti \| A. Fortunato \| T. Trenti \|

Biochimica Clinica ; 39(6) 546-547 Editoriale - Editorial

Ottimizzazione di un metodo di misura della lipasi pancreatica con 1,2-o-dilauril-rac-glicero-3-glutarico (6-metilresorufina) estere come substrato
Optimization of a 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6-methylresorufin) ester method for the measurement of pancreatic lipase catalytic activity.
L. Politi \| F. Ceriotti \|
<p>The measure of the catalytic activity of lipase in serum is a good indicator of acute pancreatitis. Today there is not an accepted reference method. Aim of this work was to optimize a method for measurement of catalytic activity of pancreatic lipase in serum, which could have the necessary characteristics to serve as a candidate reference method. Serum pools with different concentrations of lipase in native and inactivated form (heat inactivation at 56 °C for 1 h) and control materials were used as samples. Optimal concentrations in the final reaction mixture were obtained through a series of experiments. Optimization was reached when higher catalytic activity and lower nonspecific signal were obtained. The following conditions were tested: buffer type and concentration, concentration of bile salts (taurodeoxycholate and deoycholate), concentration of calcium chloride, effect of different surfactants, substrate concentration, colipase concentration, and pH. The optimization experiments lead to the following reaction conditions (concentrations in the final reaction mixture): pH, 8.0; taurodeoxycholate, 21,9 mmol/L; deoxycholate, 5,28 mmol/L; calcium chloride, 6,43 mmol/L; colipase, 2,24 mg/L; 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6-methylresorufin) ester, 0,11 mmol/L; Triton X-100, 0,75 g/L. Using the herein described measurement conditions, CV ranging from 1,4% to 2,9% were obtained. A comparison with the assay used in our clinical laboratory using the same substrate gave the following regression equation: y=1.56x + 2,4 U/L, r2=0.982.</p>
Biochimica Clinica ; 36(3) 177-186 Contributi Scientifici - Scientific Papers