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Editor-in-chief
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada


Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091



BC: Articoli scritti da E. Milletti

Caratterizzazione dei calcoli urinari mediante spettroscopia Raman e spettroscopia infrarossa in trasformata di Fourier a confronto col metodo chimico-colorimetrico
Characterization of urinary stones using Raman and Fourier transform infrared spectroscopy compared to a chemical colorimetric method
<p>Introduction: Chemical-colorimetric methods for kidney stones identification are widely adopted in most routine laboratories due to low cost with no need of instrumental analysis. Actually, kidney stones are well characterized with the Fourier transform infrared spectroscopy combined with Attenuated Total Reflection (ATR/FT-IR) that allows quick identification of chemical compounds into solid samples. Furthermore, Raman spectroscopy is under investigation as a complementary technique to ATR/FT-IR.<br />Methods: the comparison between chemical-colorimetric method and ATR/FT-IR and Raman spectroscopy has been performed evaluating standards (n=60) and urinary stones (n=89), after morphological and chemical characterization by Raman spectroscopy.<br />Results: identification rate of low quantities in standards (5% and 10% respectively) for the three methods were as follow: chemical-colorimetric method, 20% and 40%; Raman spettroscopy, 87% and 90%; ATR/FT-IR, 53% and 63%. The comparison of the 89 urinary stones revealed that chemical-colorimetric method results differ from spectroscopic technique identifications particularly for oxalate ions, phosphate and uric acid (15 samples out of 89). The agreement between the investigated methods is more than 80% in kidney stones with one prevalent compound (56 samples). In the other samples characterized by two or more compounds (33 samples), discordant identifications between methods were evaluated and discussed.<br />Discussion: ATR/FT-IR and Raman spectroscopy present many advantages in terms of identification of the most representative compounds of kidney stones; therefore, they represent a valid solution for a second level laboratory that receives a large number of samples and should examine them with the most adequate technological support.</p>
Biochimica Clinica ; 45(4) 365-374
Contributi Scientifici - Scientific Papers
 
Ruolo del laboratorio nella valutazione di un donatore di organi con sospetta emofilia A
Role of the laboratory in the evaluation of an organ donor with reported haemophilia A
<p>The case concerns a 82-year-old patient, organ donor, affected by diabetes mellitus, hypertension and reported type A haemophilia, showing a traumatic severe cerebral haemorrhage. The Medical Committee started the donor evaluation process: the liver was compatible for a recipient in life-threatening conditions. Although the first level coagulation tests were within the normal range, the Regional Center for Organ and Tissue Allocation of the Tuscany Region - Italy requested further investigations in order to clarify the reported diagnosis of haemophilia and to exclude the presence of a specific FVIII inhibitor. FVII activity was evaluated to assess the protein synthesis of the liver, and FVIII for suspected haemophilia; both of them were normal. Considering the importance of the diagnosis, the parallelisms of both FVII and FVIII were performed; the tests were negative for the presence of inhibitors. Second-level tests therefore rejected the diagnosis of haemophilia and excluded the presence of a specific inhibitor of FVIII. The absence of coagulative alterations allowed the liver explant, which was successfully transplanted on a 59-year-old male recipient.</p>
Biochimica Clinica ; 42(3) e37-e39
Casi clinici - Case report