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Maria Stella Graziani

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Giuseppe Agosta

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da B. Gini

Un approccio proteomico per l’identificazione di autoantigeni nell’encefalopatia di Hashimoto
A proteomic approach to identify autoantigens in Hashimoto’s encephalopathy
A. Farinazzo  |  B. Gini  |  G. Moretto  |  B. Bonetti  | 
<p>Hashimoto&rsquo;s encephalopathy (HE)&nbsp;is a syndrome involving the central nervous system (CNS), characterized by an heterogeneous clinical presentation with&nbsp;neurological and/or neuropsychiatric symptoms associated with high titers of anti-thyroid antibodies. Although the&nbsp;pathogenesis of HE is still unclear, the response to steroid treatment suggests that autoimmune mechanisms may be&nbsp;involved. To date, the role of anti-thyroid IgG as well as the identification of the antigen(s) targeted by IgG remain&nbsp;unknown. We performed a proteomic study on 19 patients with HE and 15 controls based on bidimensional&nbsp;electrophoresis (2D) of human CNS proteins followed by immunoblotting with cerebrospinal fluid (CSF) of patients. The&nbsp;comparative analysis of 2D maps showed that CSF IgG from HE patients specifically recognized 3 spots in a range of&nbsp;pH from 5 to 7 and of MW from 31 to 37 kDa amongst a wide autoreactivity to neural antigens. After mass spectrometry&nbsp;analysis and immunoblotting with specific antibodies, these proteins were identified as dimethylargininase-I (DDAHI) and&nbsp;aldehyde reductase-I (AKRIAI). Almost 90% of HE patients targeted at least one of two isoforms of DDAHI, while few&nbsp;cases recognized AKRIAI. The present findings suggest DDAHI and AKRIAI as biomarkers of HE although further&nbsp;experimental evidence is needed to understand their pathogenetic and diagnostic role, if any. Our 2D proteomic&nbsp;approach appears to be a sensitive and reliable method to assess the autoimmune repertoire in HE, helping to identify&nbsp;potential autoantigens in CSF and sera of HE patients.</p>
Biochimica Clinica ; 38(3) 222-226
Contributi scientifici - Scientific Papers