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Editor-in-chief
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da C. Galliani

Evaluation of Sysmex XE-2100 for enumeration and differentiation of cellular elements in peritoneal and pleural fluids

S. Buoro \| C. Ottomano \| R. Gustinetti \| C. Galliani \| E. Lochis \| C. Saleri \| P. Dominoni \| G. Lippi \|
<p>The aim of this study was to evaluate the performance of Sysmex XE-2100 for automated flow cytometric analysis of<br />biological fluids. The results of 106 consecutive peritoneal samples and 20 pleural samples were compared with optical microscopy. A good agreement was found in total nucleated cells (TNC) count between Sysmex XE-2100 and manual microscopy (y=0.98x+3.2, r=0.99; mean bias, -9.5 TNC/μL). The percentage of cells with high fluorescence in XE-2100 correlated with that of macrophages and mesothelial cells at microscopy (r=0.67; P <0.001). The CV of TNC determination on XE-2100 ranged between 1.6% and 11.7%. At the 20% CV, the analytical sensitivity of XE-2100 was 29/μL for TNC, 50/μL for polymorphonuclear leukocytes (PMN) and 66/μL for mononuclear elements. The linearity between 30 and 944 TNC/μL was excellent (r=0.998, P <0.001), whereas the carry-over was <1%. At thresholds of ≥250 PMN/μL in peritoneal fluid and ≥50% PMN or ≥50% lymphocytes in pleural fluid, the diagnostic accuracy of XE-2100 was 96.8%, with Cohen's kappa of 0.90 (P <0.001). We conclude that automated flow cytometric analysis on Sysmex XE-2100 may be a viable options for screening of peritoneal and pleural fluids.</p>
Biochimica Clinica ; 37(4) 275-278 Contributi Scientifici - Scientific Papers