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Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da P. Franceschini

The consumption of alcohol and cocaine among drunk-drivers: retrospective data analysis of a protocol for driving license regranting program
<p><span style="font-size:9pt">Introduction: alcohol and cocaine are frequently used together; this co-abuse compromises the physical and mental requirements included in the assessment of fitness to drive. The assessment for the issuing of the certificate of fitness to drive is released by Local Medical Commissions after clinical examination and toxicological analysis. The aim of this paper is to estimate the prevalence of cocaine use among drunk-drivers during a specific driving regranting protocol, in order to assess the usefulness of the inclusion of cocaine metabolites in hair analysis in this category of subjects.&nbsp;</span><br /><span style="font-size:9pt">Methods: according to the toxicological protocol at the Regional Reference toxicological laboratory of the &ldquo;Liguria Levante&rdquo; in Sarzana (La Spezia) at San Bartolomeo Hospital, in addition to the quantification of carbohydrate-deficient transferrin (CDT) in serum by HPLC and Ethylglucuronide (ETG) in keratin matrix, also cocaine and its metabolites, especially Coca-ethylene (CE) has been tested in hair by LC-MS/MS. The protocol has been applied on biological samples collected from 2 215 drunk-drivers.</span><br /><span style="font-size:9pt">Results: the 6.6% of the 2 215 hair samples analyzed were classified as non-moderate alcohol users (ETG &gt;30 pg/mg). Among these 0.1% were cocaine users and 0.9% consumed together alcohol and cocaine, as highlighted by the presence of CE. Out of the 94.4% of the 2 215 hair samples with ETG &lt;30 pg/mg, 3.9% and 6.1% were positive for cocaine, and CE respectively.</span><br /><span style="font-size:9pt">Conclusion: This study demonstrates the importance of toxicological analysis of drugs of abuse in hair and the need to include the analysis in specific protocols for driving regranting, such as the one here adopted.</span></p>
Biochimica Clinica ; 46(3) 231-234
Contributi Scientifici - Scientific Papers
Titolazioni dei preparati galenici oleosi a base di cannabis in Regione Liguria: progetto sperimentale dei laboratori di riferimento regionale
Analysis of cannabinoids concentration in cannabis oil galenic preparations in the Liguria Region: experimental project of the regional reference laboratories
<p>Introduction: the medical use of cannabis is increasingly being applied in the treatment and support of several diseases and syndromes. In the Liguria Region, olive oil galenic preparations are mainly prepared by hospital pharmacies, according to common standard procedures. The preparations must be analyzed in order to establish the concentration of the two main active compounds (delta-9 tetrahydrocannabinol, THC and cannabidiol, CBD) thus allowing the correct setting of the therapeutic prescription. Liguria Region is at the forefront in the use of medical cannabis with a high number of patients treated (&gt;1000). The aim of this work is to describe the organization of the titration activity centralized at the two regional reference laboratories (Central Laboratory of Analyses of Giannina Gaslini Institute, Genova and Toxicology Laboratory of Sarzana, La Spezia), coordinated by the inter-hospital department (DIAR) of the Laboratories Area.<br />Methods: the phases of the analytical process (pre-analytical, analytical and post-analytical) have been identified and described. The analysis of the workflow has been carried out including the methods to prepare cannabis oil in the pharmacies, the intervals and production volumes, the medical-legal handling requirements and operational responsibilities. The definition of the pre-analytical phase foresees the methods of packaging, transport and recording of the samples and related responsibilities.<br />Results: the analytical phase included the development and validation of the analytical method Ultra High Performance Liquid Chromatography coupled to tandem mass spectrometry, (UHPLC-MS/MS) in the two laboratories, with common procedures and the comparison of results conducted both on reference material and real samples of olive oil galenic preparations. The definition of the post-analytical phase included the reporting procedures.<br />Discussion: the experimental phase has been concluded at the end of 2019 and the implementation phase of the project has started in march 2020.</p>
Biochimica Clinica ; 44(4) 367-373
Contributi Scientifici - Scientific Papers