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Editor-in-chief
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada


Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091



BC: Articoli scritti da A. Farinazzo

Un approccio proteomico per l’identificazione di autoantigeni nell’encefalopatia di Hashimoto
A proteomic approach to identify autoantigens in Hashimoto’s encephalopathy
A. Farinazzo  |  B. Gini  |  G. Moretto  |  B. Bonetti  | 
<p>Hashimoto&rsquo;s encephalopathy (HE)&nbsp;is a syndrome involving the central nervous system (CNS), characterized by an heterogeneous clinical presentation with&nbsp;neurological and/or neuropsychiatric symptoms associated with high titers of anti-thyroid antibodies. Although the&nbsp;pathogenesis of HE is still unclear, the response to steroid treatment suggests that autoimmune mechanisms may be&nbsp;involved. To date, the role of anti-thyroid IgG as well as the identification of the antigen(s) targeted by IgG remain&nbsp;unknown. We performed a proteomic study on 19 patients with HE and 15 controls based on bidimensional&nbsp;electrophoresis (2D) of human CNS proteins followed by immunoblotting with cerebrospinal fluid (CSF) of patients. The&nbsp;comparative analysis of 2D maps showed that CSF IgG from HE patients specifically recognized 3 spots in a range of&nbsp;pH from 5 to 7 and of MW from 31 to 37 kDa amongst a wide autoreactivity to neural antigens. After mass spectrometry&nbsp;analysis and immunoblotting with specific antibodies, these proteins were identified as dimethylargininase-I (DDAHI) and&nbsp;aldehyde reductase-I (AKRIAI). Almost 90% of HE patients targeted at least one of two isoforms of DDAHI, while few&nbsp;cases recognized AKRIAI. The present findings suggest DDAHI and AKRIAI as biomarkers of HE although further&nbsp;experimental evidence is needed to understand their pathogenetic and diagnostic role, if any. Our 2D proteomic&nbsp;approach appears to be a sensitive and reliable method to assess the autoimmune repertoire in HE, helping to identify&nbsp;potential autoantigens in CSF and sera of HE patients.</p>
Biochimica Clinica ; 38(3) 222-226
Contributi scientifici - Scientific Papers