Member area login
You don't have or don't remember the password!
Click Here
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282


ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da F. De Liso

L’interferenza dell’emolisi sulla misura dell’insulina
Analytical interference of hemolysis on serum insulin measurement
<p>Insulin is a polypeptide hormone, secretedfrom pancreatic &beta;-cell, involved in the regulation of glucose and lipid metabolism. Insulin measurement is a useful toolto identify some clinical conditions, such as fasting hypoglycemia, some types of diabetes, the presence of insulinresistance. An important pre-analytical aspect that influences the determination of insulin serum concentration is thepresence of hemolysis. In fact, it is well known that insulin is degraded by a protease released by red blood cells afterhemolysis. The aim of this study is to evaluate the interference of hemolysis on insulin measurements performed bya chemiluminescence method. To study the effect of hemolysis on insulin degradation, we added increasingconcentrations of red blood cell hemolysate to a serum pool with known insulin concentration. The reduction of insulinlevels was affected by the degree of hemolysis, by the time elapsed before the assay and by the temperature ofsamples storage. Our results show that insulin values do not decrease significantly (&lt;10%) when hemolysis is &lt;2.0g/L of free hemoglobin (corresponding to H-index=200) if samples are maintained at low temperatures (i.e. in an ice-water slurry) until the assay is performed.</p>
Biochimica Clinica ; 43(3) 273-277
Contributi Scientifici - Scientific Papers
Una diagnosi in laboratorio
A case of atypical celiac disease with hematological involvement
<p>We report a case of a pregnant 39 year-old woman (22th week) with leuco-granulocitopenia, iron-deficiency anemia and a slight elevation of transaminases, lasting for several years. The origin of these biochemical alterations was unclear. Assuming a possible autoimmune condition, she underwent a hematological counselling in order to verify the presence of an immunological disorder. Anti-nuclear antibodies resulted negative with a citoplasmic filamentous fluorescence suggestive of anti-smooth muscle antibodies (ASMA). On a suitable substrate for ASMA detection, a reticulin-like fluorescence was observed, inducing to investigate for the presence of celiac disease (CD). CD diagnosis requires both the biochemical tests and duodenal biopsy; however, because of the pregnancy only the serum panel was performed (serum IgA, anti-tissue transglutaminase IgA, anti-endomisium IgA). As the tests were all positive, clinicians decided to start the gluten free diet with an improvement of the hematological and biochemical parameters. The patient gave birth to a healthy newborn.</p>
Biochimica Clinica ; 42(1) e08-e011
Casi clinici - Case report