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Editor-in-chief
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Publisher
Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da A. Coglianese

Effects of selected preanalytical variables on Dried Blood Spot (DBS) and Volumetric Adsorptive Microsampling (VAMS) based bioanalytical methods for the determination of four β-lactam antibiotics

F. Mensitieri \| A. Coglianese \| V. Giudice \| B. Charlier \| F. De Rosa \| F. Filippelli \| F. Dal Piaz \| V. Izzo \|
<p><span style="font-size:9pt">Introduction: dose adjustment of antibiotic drugs is fundamental in critically ill patients and newborns, who may experience inadequate exposure of these drugs due to different pharmacokinetics (PK) and pharmacodynamics (PD) compared to other patients. Despite the increasing use of microsampling devices such as Dried Blood Spot (DBS) and Volumetric Absorptive Microsampling (VAMS) in paediatric clinical practice, little is reported on the comparison between these two devices in terms of β-lactam antibiotics quantification.</span><br /><span style="font-size:9pt">Methods: a method using high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of amoxicillin, ampicillin, meropenem and cefadroxil from both DBS and VAMS has been evaluated. More specifically, we investigated the influence of some well-known critical pre-analytical parameters, such as blood haematocrit, blood spot volume and sample recovery approach on the results obtained with DBS-based and VAMS-based analytical methods.</span><br /><span style="font-size:9pt">Results: our results show that comparable trueness and precision values can be achieved using both DBS- or VAMS-based methods. However, the latter was not affected by blood haematocrit value, whereas accuracy of the DBS-based approach was influenced by parameters as haematocrit, blood spot approaches (by pipette or hanging drop) and spot recovery procedures (DBS cut or punch). For the β-lactams tested, a significant longer stability was found for samples absorbed on DBS, when samples were stored at 4°C.</span><br /><span style="font-size:9pt">Discussion: our data support the use of VAMS as preferable devices for β-lactam determinations, while more attention needs to be devoted to different pre-analytical variables for an accurate DBS quantification.</span></p>
Biochimica Clinica ; 46(2) 141-153 Contributi Scientifici - Scientific Papers

Armonizzazione in Medicina di Laboratorio
Harmonization in Laboratory Medicine
F. Ceriotti \| M. Panteghini \| A. Tosetto \| V. Valentini \| L. Politi \| R. Rolla \| T. Guastafierro \| T. Köken \| E. Capoluongo \| C. Mazzaccara \| V. D'Argenio \| V. D'Argenio \| G. Lippi \| M. Plebani \| D. Giavarina \| M. Berardi \| A survey on sample matrix and preanalytical management in clinical laboratories \| D. Bozzato \| G. Messeri \| M. Zaninotto \| M. Vidali \| A. Padoan \| G. Parigi \| A. Clerico \| L. Sciacovelli \| M. Ciaccio \| G.L. Salvagno \| G. Barberio \| G. Barberio \| G.L. Salvagno \| M. Panteghini \| F. Braga \| G. Gessoni \| M. Montagnana \| N. Doğan \| M. Barberis \| M. Barberis \| A. Marchetti \| F. Borrillo \| L. Bonfanti \| P.M. Ness \| G. Messeri \| S. Nannini \| J. Queraltò \| M. Zaninotto \| A. Mosca \| BM. Henry \| G. Santini \| A. Coglianese \| E. Fiorio \| L. Crinò \| M. A. V. Willrich \| A. Modenese \| M. Berardi \| G. Nordera \| M. Girelli \| R. Tomaiuolo \| D. Giavarina \| R. Dittadi \| L. Pighi \| R. Danesi \| M. Locatelli \| F. Balboni \| D. Cosseddu \| M. Savoia \| S. Bernardini \| C. Domenichini \| M. Lamonaca \| M. Perrone \| M. Perrone \| per il Gruppo di Studio Intersocietario SIBioC-SIPMeL Diabete Mellito \| P. Pradella \| A. Padoan \| L. Belloni \| A. D'Avolio \| T. Trenti \| A. Fortunato \| T. Trenti \|

Biochimica Clinica ; 39(6) 546-547 Editoriale - Editorial