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Maria Stella Graziani

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ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

BC: Articoli scritti da M. Borsotti

Stabilità delle principali droghe d’abuso in campioni di urine non trattate rispetto a campioni di urine stabilizzate
Drug of abuse stability in native urine specimens vs. stabilized urine samples
<p>Effective urine drug of abuse testing&nbsp;requires an understanding of the stability of drugs and their metabolites excreted in the urine matrix. As timing of&nbsp;specimen collection is crucial in urine screening of drugs, potential degradation that may occur between, e.g., the initial&nbsp;test and the re-test of samples, should be considered. Stability characteristics depend on the drug, specimen&rsquo;s pH,&nbsp;storage temperature, light exposure, bacteria contamination and material of the urine container. The purpose of this&nbsp;study was to determine the stability in urine specimens of four classes of drugs and their metabolites (cannabinoids,&nbsp;opiates, cocaine and methadone) for a maximum of 20 weeks. In the study design, a freshly authentic pooled urine&nbsp;specimen was aliquoted into vials (plastic and glass) and these were stored at freezing (-20 &deg;C), at fridge (4 &deg;C) and at&nbsp;room temperatures and reanalyzed every one/two weeks up to 20 weeks. In parallel, we extended the study analysing&nbsp;one urine pool stabilized using various techniques of thawing and filtration for preventing the decomposition of drugs of&nbsp;abuse. In the native specimens, analytes were relatively stable when stored for extended periods of time at -20 &deg;C.&nbsp;Concentrations of cannabinoids, cocaine metabolites and morphine in not-pretreated samples decreased over time at 4&nbsp;&deg;C and at room temperature. Methadone did not show relevant loss of drug concentrations during fridge storage.&nbsp;Manufactured urine pool was stable, showing little changes between day 1 and after 10 weeks at 4 &deg;C. Morphine,&nbsp;cannabinoids and methadone showed no loss in drug concentrations after two weeks at room temperature, while&nbsp;benzoylecgonine concentrations significantly decreased after one week.</p>
Biochimica Clinica ; 38(2) 103-109
Contributi scientifici - Scientific papers